Analyzing Cell Motion II

This week we will be learning how to use Fiji to analyze videos of cell motion. The Scenario: A patient has a wound, in the process of healing, that is infected with bacteria. Will the patient need antibiotics? To explore this scenario, you will be analyzing videos of: 1) wound healing, 2) neutrophil (white blood cells) motion, and 3) bacteria motion. Clearly, the relative speeds of the neutrophils (white blood cells), and bacteria will affect your decision. You have been provided with three videos: Wound healing, neutrophil motion, and bacteria motion. Each video is a sequence of images called ‘frames.’ The wound healing videos, ‘WoundHealing,’ show breast tissue cell sheet migration. The ‘Neutrophils’ videos show white blood cells responding to six different concentrations of fMLP— the chemical indicator of bacteria. The bacteria videos show E. coli motion. These videos are rich in detail but the files contain too much data to be analyzed in our limited lab time. From the shorter videos, your task is to perform a quantitative analysis, with Fiji and Excel, of the rates of motion of these cells. T At the end of the lab today, you and your partner (or you individually) will submit a lab report, including tables for all three videos, the calculation of average velocity for both the neutrophils and the E. coli bacteria using your data, and a paragraph explaining whether the patient needs antibiotics and why. Be sure to include an explicit comparison of the white blood cell and E. coli velocities. You will upload these as one PDF report to the assignment link on canvas. Video Files: o Wound healing: WoundHealing_25fps.avi; (Technical specifications: 0.65 μm/pixel, 6.0 min/frame, playing at 25 frames/sec) o White blood cells: Neutrophils_25fps.avi; (Technical specifications: 1.326 μm/pixel, 7.2 sec/frame, playing at 25 frames/sec) o Bacteria:E_Coli_25fps.avi; (Technical specifications: 156 pixels/μm, 0.050 sec/frame, playing at 25 frames/sec)