Biology /Protein engineering!

A startup is developing an anti-inflammatory biological drug based on the human progranulin (see homework 2 for the amino acid sequence). The chimeric protein has to have hGrnA (the third granulin repeat in the sequence) merged at its C-terminus with hGrnD (human granulin D, the fifth granulin repeat in the sequence). To facilitate DNA manipulations, the hGrnA expression vector you obtained in homework 2 has been modified in such a way that a unique XhoI recognition site (c|tcgag, used to clone hGrnA in homework 2) was replaced with a unique PvuII site (cag|ctg). You will need to digest with PvuII the vector for hGrnA expression, and insert the hGrnD sequence.

Please find the attachment HW#3 for the full assignment