Design a set of primers to clone the argB gene from Lactobacillus plantarum WCFS1 into the NdeI and BamHI sites on the pET32a(+) plasmid using traditional cut-and-past (restriction enzyme) cloning.

1. Design a set of primers to clone the argB gene from Lactobacillus plantarum WCFS1 into the NdeI and BamHI sites on the pET32a(+) plasmid using traditional cut-and-past (restriction enzyme) cloning. You can find the sequence for the argB gene at the following link: https://www.ncbi.nlm.nih.gov/gene/1061853.

2. Design a set of primers to clone the argB gene from Lactobacillus plantarum WCFS1 into the NdeI and BamHI sites on the pET32a(+) plasmid using Gibson Assembly.

3. GeneX was cloned into the pET32a(+) plasmid using NdeI and EagI restriction enzymes. Successful cloning was verified by DNA sequencing using the T7 and T7term primers. You can find the sequencing data on Blackboard under the lecture folder for 2/27/20. a. Determine the sequence of GeneX. b. What is GeneX? (Hint: use BLAST) c. How does GeneX compare with similar enzymes in the Genbank? (Hint: use Clustal Omega)